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This limitation arises from the use of the ESI-MS/MS technique based on the in-solution buffer-free digestion method employed in this study to characterize RBD variants, which is not currently quantitative for such analysis ( In short, S-alkylation of C538 in the RBD protein leads to multiple chemical modifications resulting from free cysteines being exposed

10.3389/fncel.2023.1328269 Front

The physiological regulation and cross-talk among individual hydrolases remains largely uncharted investigative territory and is likely to reveal important new roles in signalling and neuronal dysfunctions more subtle than degeneration 239,240

Embo J 41(7):e109169

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One category, encompassing about one-third (36%) of all phosphorylation sites, scored in the 90th percentile or better for six or more conventional Tyr kinases, indicating predicted favourability to a broad spectrum of kinases